Kjeldahl method | Nitrogen Determination, Protein Analysis & Volumetric Analysis (2024)

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Kjeldahl method | Nitrogen Determination, Protein Analysis & Volumetric Analysis (1)

Kjeldahl method, in analytical chemistry, procedure widely used for estimating the nitrogen content of foodstuffs, fertilizers, and other substances, invented in 1883 by a Danish chemist, Johan G.C.T. Kjeldahl. The method consists essentially of transforming all nitrogen in a weighed sample into ammonium sulfate by digestion with sulfuric acid, alkalizing the solution, and determining the resulting ammonia by distilling it into a measured volume of standard acid, the excess of which is determined by titration.

This article was most recently revised and updated by Michele Metych.

Kjeldahl method | Nitrogen Determination, Protein Analysis & Volumetric Analysis (2024)
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